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Ten kilobases of sequence immediately adjacent to the end of hrpF are unique to these strains.
A Dictyostelium line containing a single loxP site adjacent to the 3' end of the sec1A gene was engineered.
G418-resistant ES cell colonies were picked individually at day 10 of selection (0.4 mg/ml G418) and clones with a targeted Pink1 gene were identified by PCR with primer P1 (5'-attgctgaagagcttggcggcgaatgggct-3') located in the neo gene and primer P2 (5'-tgc tgactgctgcaagagccaggcgatca-3') located in Pink1 exon 8 adjacent to the 3' end of the targeting vector (shown in Figure 1).
Our design incorporates an additional mismatched nucleotide pair placed adjacent to the end of the stem (ACUCGAG A, mismatches indicated in bold).
For each gene considered, either another gene or a repetitive sequence was adjacent; if a gene was adjacent to the end of a scaffold, it was categorised as "repetitive sequence adjacent".
The sequences adjacent to the end of the CRISPRs were conserved on both sides of the CRISPRs; it was difficult to find a difference in AT-richness between the adjacent sequences on both sides.
Similar(39)
The 5' and 3' ends of both rrnL and rrnS genes were assumed to be adjacent to the ends of bordering tRNA genes.
The 5' and 3' end of 12S rRNA genes are defined as immediately adjacent to the ends of the flanking genes tRNA Phe and tRNA Glu, in the two Acanthaster mtDNA genomes.
For example, a decoy with five pokes that are adjacent to the ends of the closed loops that they are poking is given a poke cost of 1.39, which is less than 2.03 (the poke cost for a decoy containing a co-poke), whereas the decoy with five pokes would have a cost of 3.45 had we summed the log scores.
The interchange also serves as the southern terminus of NY 15 and is adjacent to the north end of NY 415, which terminates north of the exit at NY 21.
ESEs placed adjacent to the 3′ end of the hairpin structure display high enhancer activity, regardless of their sequence identities.
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