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Immunoreactive species were quantified in digitized images of replicas using Image J; the background signal from an adjacent, identically-sized area in the identical lane was subtracted from each reactive species (Yabe et al., 2001).
A negative control was stained along with each test section and consisted of the adjacent section treated identically except that no primary antibody was applied.
The increase in CGRP release evoked in pairs of identically stimulated adjacent slices was correlated (Capsaicin 100 nM, R = 0.64, p = 0.004, n = 19 pairs from 11 animals).
Next, we created identically sized spherical ROIs.
They were identical twins but didn't photograph identically.
We previously suggested that pairs of adjacent a and bg genes (i.e. those that are identically numbered) might be co-expressed in single tissues or organs [ 19].
The concordance of adjacent CpGs in the same read was defined as the ratio of the number of identically methylated (or unmethylated) CpG pairs over the total number of CpG pairs.
Human muscle biopsies (Table 2) were processed as above, and immunostaining was performed identically, except that GCS and TDP-43 antibodies (Proteintech), both diluted 1/100, were applied to adjacent sections, respectively, to ensure that their immunoreactivities could be seen within the same myofibers.
For such calculations, we consider adjacent abnormal SNPs to be more likely of biological origin than error or chance and treat non-adjacent abnormal SNPs identically regardless of biological or technical origin.
(l) identically, adding par.
Adjacent, Nos.
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