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Here the sum of the denominator and numerator of the third fraction of the primary series gives the denominator 7 to the third fraction of the secondary series, and so on ; and, as in the primary series, so in the others, the sum of the denominators of two adjacent fractions gives the denominator of the next succeeding.
Similar factors were calculated between adjacent fractions (e.g., F1 vs F2), using cell number and the proportions of cells in each phase, as determined by flow cytometry.
In the set of 1709 genes, a peak fraction was assigned for 1317 genes: 1288 genes had a peak contained in a single fraction, 11 genes had a peak which overlapped two adjacent fractions, and 18 genes were found with a peak extending over three adjacent fractions.
To save the machine time, we first checked the peptide quality and abundance in each fraction by MALDI-TOF, and combined the adjacent fractions with little peptides, resulting in a total of 18 fractions for ESI-Q-TOF detection.
Adjacent fractions with largely overlapping peptide compositions were pooled (Supplementary Figure S1 in Additional file 1), giving a total of 29 fractions with different sets of citrullinated fibrinogen peptides that were selected for iSPR analysis.
When the 95% bootstrap confidence interval was not confined to a single fraction, the definition of the peak fraction was widened from only one to two (or more) adjacent fractions and the search for the maximum was started again.
Similar(51)
The hSAA protein was measured in plasma from hSAA transgenic mice, in pools of two adjacent FPLC fractions, and in ultracentrifugation samples using a Human SAA ELISA Kit (Biosource, Camarillo, CA).
Some peptides were observed in adjacent HPLC fractions because of peak tailing and possible random error caused from manual collection.
Summation of adjacent recombination fractions was calculated as illustrated by Liu et al. The annealing system continued until, in a number of successive steps, the newly generated map order is rejected.
Marker order was determined with the AutoMap function and different combinations of the Branch and Bound II and Seriation II ordering algorithms with Sum of Adjacent Recombination Fractions and Count criteria for ordering and rippling.
The sum of adjacent recombination fractions (SARF), a more sensitive indicator of map expansion caused by suboptimal marker positioning, revealed a minor degree of expansion of 5.2 ± 2.9% (mean ± SD across populations).
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