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A volume of 100 µL was transferred into each well of a 96-well plastic plate (Nunc, NY) and sealed using a transparent adhesive seal.
Briefly, one hundred microliters of log-phase grown cultures adjusted to an OD600 of 0.05 in MH broth was added to each well in the Sensititre® susceptibility plate and the wells were covered using the perforated adhesive seal.
The plates were sealed with a polypropylene adhesive seal (Titer-Tops, Diversified Biotech) augmented with custom magnetic sealing lids.
To avoid evaporation, each utilized MTP were sealed with a gas-permeable adhesive seal (ThermoScientific, Waltham, USA) for the cultivations in the BioLector prototype.
The plate was sealed with adhesive seal and centrifuged at 240 g for 20 sec at room temperature to ensure the contents would contact the bottom of the well.
The cultures were stored at -20°C in 100 μL aliquots in 96-well MTPs, which were closed with an adhesive seal (Cat. AB-0580, Thermo Scientific, Karlsruhe, Germany).
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Then he used a lighter to warm the razor, which he wedged under the adhesive sealing the booklet, and peeled back the tab.
Originally scheduled for 2 October, the rerun had to be postponed by two months after the adhesive seals on postal votes were found to have come unstuck.
A multiplate 96-well format with adhesive sealing was used for the analysis (Bio-Rad).
The plates were sealed with gas permeable adhesive seals and the seed cultures grown overnight at 37°C with shaking at 250 rpm.
The microtiter plates were sealed with sterile gas-permeable adhesive seals (Thermo Scientific, Waltham, MA, USA), ensuring sterile conditions in the wells and allowing good ventilation.
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