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Cell adhesion ratio ranked from excellent (close to 100%) to satisfactory (around 50%) in the order PCL/GEL > CS/GEL > CS/PCL/GEL > CS/PCL.
Based on this analysis, the relationship between the shear strain and design parameters, such as the rubber adhesion ratio, pressure difference, elastic modulus of stator, magnitude of interference and wall thickness, is investigated.
Up to now, most investigations of friction modifiers were conducted in the field, where it is difficult to control or measure important parameters such as angle of attack, rolling speed, adhesion ratio etc.
Furthermore, we computed the adhesion ratio as the ratio of adherent conidia over all macrophage-associated conidia.
Presence of these genes suggested the possibility of specific interaction and colonization potential of Lp9 isolate in the gut, which was also suggested by a good adhesion ratio of 7.4±1.3% with Caco-2 cell line.
In Fig. 5 B, it can be seen that the mean value of the adhesion ratio is 45% lower for the pksP mutant conidia than for the wild-type conidia.
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In addition to the determination of the phagocytosis and adhesion ratios our method provides results on the aggregation behaviour of conidia, as can be seen in Fig. 6.
Second, figures in previous report were obtained using non-confluent ME180 cells, whereas adhesion ratios reported here were obtained with cells at higher densities (sub-confluent).
Instead, we calculated adhesion ratios comparing the number of CFU recovered from cell-associated bacteria to the total amount of bacteria in the well, at the corresponding time-point.
The statistical analysis is summarised in Table 6 and reveals that the difference in both phagocytosis and adhesion ratios is highly significant ( <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0019591.e076.PNG" class= inline-graphic"/> ).
All three tests verify the accuracy of the assumption for a normal distribution of the phagocytosis and adhesion ratios, as required for the application of the Welch t-test.
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