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The bacterial suspensions were then cultured at 37 °C for 18 h at a 30° angle and sucrose-dependent adhesion analysis was performed as previously described24.
Focal adhesion properties were analysed using the focal adhesion analysis server53 (three biological repeats; over 33 movies per condition analysed; adhesion lifetime and maximal area, n>112,000 adhesions analysed; assembly rate, n>6100 adhesions analysed; disassembly rate, n>7,200 adhesions analysed, ***P value <1.73 × 10−50).
In the present work, injection-molding mold-filling and material-packing analyses are combined with a structural analysis involving polymer/metal adhesion analysis to assess the extent of residual stresses and warping in a prototypical direct-adhesion PMH component.
Consequently, the cotton yarn obtains higher wicking heights and smaller contact angles at lower MRs. The size adhesion analysis proves that the adhesion between the roving and the size becomes weaker as the MR increases.
The relatively uniform dispersion of nHA-I in the PLGA polymer matrix was beneficial for the osteoblastic cell adhesion analysis because one portion of the nHA-I was embedded while the rest protruded from the electrospun PLGA/nHA-I composite nanofibers surface.
Both for cell spreading and focal adhesion analysis MEFs were re-plated at a density of 100,000 cells per 6-well dish on fibronectin-coated coverslips.
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Bioactivity, cytotoxicity and cell viability were assessed via MTT assay with 2 cell cultures and cell spreading-adhesion analysis.
Nagafuchi, A., Ishihara, S. & Tsukita, S. The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules.
For the spreading assay and subsequent adhesion site analysis, cells were prepared as described above prior to seeding onto glass coverslips coated with 50 μg/mL FN.
BS performed the in vitro assays (cell adhesion assays, FACS analysis, reporter gene assays) and the statistical analysis.
Effects of tail design and hybrid adhesion with force analysis are numerically evaluated and presented.
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