Its adherents point out that the witness, Mike McQueary, has testified that out of respect for Paterno's age and status, he used mild, perhaps ambiguous language when describing what he saw to the coach.
At the indicated time points, adherent and non-adherent cells were collected, washed with PBS, fixed in 70% ethanol and stored at 4°C until use.
At various time points, adherent and non-adherent cells were recovered, washed twice in PBS, fixed in 70% ethanol, and stored at 4°C until analysed.
Consistent with its key role in anoikis, Bim was undetectable in these adherent culture time points (data not shown), further supporting the notion that loss of focal adhesions and decreased adherence precede Bim upregulation.
At the indicated time points, adherent peritoneal macrophages from T. crassiceps-infected MGL1+/+ and MGL1−/− mice were aseptically removed and without any further stimulation (basal) or LPS + IFN- γ-stimulation (1 μg and 20 ng/mL, resp).
Even though daily adherence could be improved in initially non-adherent patients, it has to be pointed out that this patient population did not reach the same adherence level as initially adherent patients.
At different time points, the adherent cells were stained on chamberslides with fluorescein-conjugated annexin V and propidium iodide using Annexin-V-FLUOS Staining Kit (Roche) as per the manufacturer's instructions and the fluorescence of individual cells was assayed by fluorescence microscopy.
b Angled view within the hernial sac (inguinal canal) using the 30° laparoscope, black arrows pointing to adherent ascending colon.
Despite the carefully managed nature of the Multimodal Treatment Study of Children with ADHD (MTA), saliva assays for MPH revealed that approximately 25%% of 254 patients in the medication arms of the study were non-adherent on 50 % or more of repeated assays, and that barely half (54 %) were adherent at every assay point [ 67].
Patients were defined as adherent if they scored ≥13/15 points on the adherence index (Table 1).
