Participation in the survey was voluntary and confidentiality of respondents and their responses was assured by adherence to Section 308(d) of the Public Health Service Act (42 USC 242 m).
A. Perceptions of the extent of patient non-adherence: This section contains a total of six questions split equally into two subsections.
For immunogold labelling, these latter were immersed in a solution of sellotape dissolved in chloroform prior to use, to enhance section adherence during the labelling procedure.
The tissue sections were place on Poly-L-lysine coated slides, then kept in oven at 60 (C°) for 24 hours in order to increase section adherence to the slide.
Then, the tissue was dehydrated in alcohol, included in Paraplast (Tyco/ Health Care USA), cut in sections of 5 μm each and mounted on Xylane- 3-aminopropyl) triethoXylane- 3-aminopropylch-treated slides to foster adherence of tissue sections.
Further details are summarized Table 4. a plasma concentrations below the quantification limit indicate non-adherence (see Methods section).
For PA localization in developing pea seed coats, a celloidin coating was applied to all slides prior to the staining process to improve the adherence of tissue sections to the glass slides.
After sectioning and adherence to gelatin-coated microscope slides, sections were sequentially immunostained in a TedPella Biowave microwave and antibody dilutions used in this experiment are detailed in Supplemental Table 1.
For all animal experimentation described in the manuscript, Science Translational Medicine requires that authors state in the Methods section their adherence to the NIH Guide for the Care and Use of Laboratory Animals, or the equivalent.
For all animal experimentation described in the manuscript, Science requires that authors state in the Methods section their adherence to the NIH Guide for the Care and Use of Laboratory Animals, or the equivalent.
