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Lymphocytes were separated from adherent PMN and monocytes by adherence method.
Agreement between adherence methods was assessed by Kappa statistics and Bland/Altman plots.[10] To calculate Kappa statistics, children were categorized as having adherence <80% versus ≥80% and <95% versus ≥95% for each adherence method.
Monocyte-enriched fraction was isolated from PBMCs by a commonly used plastic adherence method [26], [27] or purified by CD14-positive selection using anti-CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) with a Midi MACS separator unit according to the manufacturer's instructions.
Monocyte isolation by the adherence method was performed as described previously [ 26].
Although MEMS gave realistic adherence data in our study, no single adherence method is ideal.
Monocytes were isolated using the plastic adherence method (Zhou & Tedder, 1995).
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The purpose of this study was to analyse the diagnostic validity of indirect adherence methods.
Of the adherence methods investigated in this study, pharmacy refill method had the best performance in predicting virological failure.
When we assessed the self-reported adherence methods, these were 100% for the HST, 60.8% for the MGT and 46.9% for the BT.
When we assessed the indirect adherence methods, these were 100% for the HST, 64.1% for the MGT and 64.9% for the BT.
Sensitivity analyses will be conducted with regard to thresholds for adherence, methods of handling missing data and fixed-effects meta-analysis.
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