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The differences in adhesion and osteoinductive capacity can be explained by the fact that DBM consists of collagen and contains a variety of adhered growth factors such as bone morphogenetic proteins (BMP) or transforming growth factor- (TGF-) β [ 34].
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After cells had adhered, the growth medium in each well was replaced with fresh medium containing NB-DNJ (0, 0.5 and 1 mM) and replenished every 24 h thereafter.
In addition, ARP-1 cells are non-adherent whereas CAG adhere to the growth surface.
A rough and porous surface can increase mechanical interlocking of an implant with bone tissue and induces adhered osteoblasts to secrete growth factors and cytokinins which subsequently increase the proliferation, differentiation, and fusion capacity of osteoblasts [ 26].
Cell seeding density of BM mononuclear cells or whole BM cells is another important factor to determine the efficiency of MSC yield as this affects adherence of MSCs, contamination by other cell types, and initial growth of adhered MSCs.
Zn deposits showed compact growth and adhered to the electrode surface.
The peptide supported LESC growth when adhered to a silicone hydrogel contact lens indicating potential use in improved LESC grafting through suppression of inflammation.
Cells were seeded in 96-well microplates (black wells with transparent bottom) at a density of 5 × 10 well−1 and allowed to adhere in the growth medium to 50 60% confluence before being exposed to the tested drugs and buthionine-sulphoximine (BSO), respectively, for 3, 6 or 27 h in quadruplicates.
Maturation of the biofilm proceeds via co-aggregation of planktonic bacteria to the already adhered biofilm [21] and bacterial growth, as has been shown for Streptococcus sanguinis [22].
After cells adhered to the wells (12h), growth medium was replaced by conditioned medium.
Approximately 5 × 10 HeLa cells were seeded onto glass-bottomed culture dishes in complete growth media, and adhered for a minimum of 24 h at 37 °C.
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