Drying in this manner resulted in separation of a fraction of the base plates from the panels and delamination of the underlying adhered layer from the calcified base plate.
In order to remove the adhered microorganisms from the surface it was vortexed for 1 min at maximum speed.
For DNA extraction from solid samples, the samples were resuspended in phosphate buffer saline and vortexed for one and half hours for dislodging the tightly adhered bacteria from the solid feed particles.
Nevertheless, regulations are followed and adhered to, from the IMO and from SOLAS, and also SOPs are followed.
Each leukocyte being tracked was followed until it adhered, detached from the endothelium, rolled out of the cremaster vasculature, or was lost due to technical limitations of the tracking procedure.
These results confirm that 1 minute of stomaching is enough to remove adhered cells from the anodized surface as well as the machined.
As the historian Ian Kershaw has observed in his biography of Hitler, this response indicates that he adhered unswervingly, from the end of World War I until his final days in the Berlin bunker, to nationalism and radical anti-Semitism.
The samples were then pre-incubated for 1 h with 20 μl protein A-sepharose CL-4B (Amersham Biosciences, Uppsala, Sweden) and centrifuged to remove any non-specifically adhered proteins from the A-sepharose.
To test this hypothesis, individual leukocytes were tracked down a venular tree in a TNF-α treated cremaster until they adhered, detached from the endothelium, or were lost due to technical limitations.
This 3-D chemical gradient encourages 3T3 fibroblast cells to adhere homogeneously from the periphery to the centre, when balanced by the tortuousity of the pore structure, which cells experience when passing from the surrounding medium to the centre.
