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The percentage, or proportion of leukocytes that adhered for each group was also studied.
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The curves in Fig. 4A separate the region where more than 50% of cells are adhered (inside each curve) from the region where less than 50% are adhered for three different scenarios.
PBMC were adhered for 2 h at 37°C in 24-well plates.
Alternatively, cells were allowed to adhere for 2 hr and then incubated for an additional 2 hr with fibronectin or 70-kDa fragment before washing and fixation, or adhered for 1 hr and incubated for an additional 3 hr.
Adhered platelets sometimes released after seconds, but most platelets adhered for longer and released after minutes.
Further guidance on NTIDs was adhered for cyclosporine, tacrolimus, and mycophenolate mofetil [ 23].
Cells were allowed to adhere for 72 h and non-adherent cells were removed with medium changes.
MC3T3-E1 cells (1.5×106 cells/ml) were added to each well and allowed to adhere for 45 minutes at 37°C.
Luciferase expressing FaDu cells were then added to each well and allowed to adhere for 30 or 60 minutes.
Tumor cells adhering for ≥10 seconds were defined as stably adherent.
Cells were allowed to adhere for 1 h before rinsing three times with fresh medium (0.5 mL each time).
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