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After the League of Nations failed as an effective international body, Koht again favoured the strict neutrality policy to which Norway had adhered before the League of Nations membership.
However, there has been ongoing discussions and frequent stakeholder's consultations focussing on how to adapt the WHO's global recommendations which provide a recipe to be adhered before attempting to scale up task shifting.
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The cells were incubated at 37°C for 4 hours to allow the cells to adhere before washing with PBS to remove the non-adhered cells.
The foam then wets the dust particles, causing them to adhere before they spread into the air, resulting in better dust control than with water sprays.
MSCs and mFBs were seeded into each chamber and allowed to adhere before stamp removal, leaving separated cell patterns on the slide.
HUVEC seeded in serum-free media in 8-well chamber slides coated with complete Matrigel (4×104 cells/well) were allowed to adhere before being sandwiched with another layer of Matrigel that was uniformly pipetted on top of the adherent cells.
Cells were allowed to adhere before adding T cells at an effector : target ratio of 20 : 1.
In co-culture experiments, fibroblasts were added first and allowed to adhere before adding tumor cells.
Briefly, FPS cells were seeded at a density of 2 × 106 cells and allowed to adhere before serum-starvation for 24 hrs.
For cytokine treatments, cells were plated and allowed to adhere before adding medium containing 10ng/ml final of IL-6 or TGF-β1 (recombinant human, Peprotech).
Cells were seeded into 96-well microtiter plates at a density of 5×10 per well and allowed 24 h to adhere before drugs were introduced (final concentration 0.1 n M 100 μ M, n=8).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com