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Cells were subsequently seeded into 96-, 24- or six- well plates and were allowed to adhere over a 24 h period.
Thus, MSCs that have been allowed to adhere over a larger area are able to differentiate towards the osteogenic lineage while cells adhering to a smaller area are restricted to the adipogenic lineage.
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Bailey also could not ignore the golden rule he adhered to over a 30-year career in which he captured virtually every major race and more than $295 million in purses.
Cells adhered over a coverslip were fixed with IC Fixation buffer (Invitrogen) for 15 min at room temperature, followed by treatment with 90% alcohol for 7 min.
The values clarification component helps individuals further clarify which risk reducing strategy they might pursue (i.e. which is most concordant with their values and would best allow them to adhere over time) by employing an explicit ranking and rating values clarification exercise.
"For weight loss to stick, you have to be able to settle into an eating pattern that you can adhere to over time," said Suzanne Havala Hobbs, a clinical associate professor at the Gillings School of Global Public Health, at the University of North Carolina, Chapel Hill.
The trajectory model offers unique opportunities to study adherence, specifically because measurable patient attributes are clearer when evaluated in the context of the work required to adhere over time.
In many cases micron-sized membranes adhere over large areas before fusion.
105 cells were seeded in 300µL medium into 24 well plates and allowed to adhere over night.
Cells were seeded at equal amounts (1000 cells for MDA-MB-231, 5000 cells for Hs578T) into 96 well plates and allowed to adhere over night.
Synthetic platelets, which mimic the size, shape, and flexibility of real platelets, were shown to adhere over 2-fold more strongly than spherical counterparts.
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