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Constructs were then inverted and the particulate-templated side was seeded with an additional 8 × 10 chondrocytes in 80 μl of expansion medium, which were allowed to adhere for an additional 2 h before returning the constructs to the upright position and adding 2 ml expansion medium.
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SF767 and MCF7 cells were plated on glass coverslips and allowed to adhere for a minimum of 24 hours.
Cells were allowed to adhere for a minimum of 8 h before MMC treatment for 24 h.
Cells were allowed to adhere for a minimum of 8 h before addition of the indicated doses of genotoxin.
Endothelial cells were pre-incubated with test peptides and VEGF for 1 h, and then allowed to adhere for a further hour (Delaney et al, 2006).
The only statistically significant findings were that municipal facilities were more likely to adhere to free care for TB than non-municipal ones; that health centres were more likely than dispensaries to adhere for a child with malaria, but dispensaries were more likely to adhere for ANC; and that facilities without laboratories were more likely to adhere for ANC.
Adherent cells are dispersed over the array as suspension and allowed to adhere for a short period, commonly 5 to 15 minutes before unadhered cells, unable to make permanent contact onto the array background during the initial adherence incubation, are washed off.
Allow the key to adhere for a few hours before moving the box or board.
Then the smile froze, adhering for a moment longer to his otherwise expressionless face as carefully, through his teeth, Raymond began to swear.
Results showed that Salmonella and Listeria species initial adhesion (2 h) is similar (Log 4.5 to 5.5 CFU/cm2), while the number of E. coli adhered for a 2 h biofilm is slightly higher (Log 5 to 6 CFigure) (Figure S6).
Approximately 5 × 10 HeLa cells were seeded onto glass-bottomed culture dishes in complete growth media, and adhered for a minimum of 24 h at 37 °C.
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