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Briefly, islets were seeded in 24-well microtiter plates (50 islets per well) and left overnight to adhere before being exposed to different concentrations of STZ and FRF.
The cells were incubated at 37°C for 4 hours to allow the cells to adhere before washing with PBS to remove the non-adhered cells.
Ideas would adhere before being broken up in cycles of contestability.
The foam then wets the dust particles, causing them to adhere before they spread into the air, resulting in better dust control than with water sprays.
MSCs and mFBs were seeded into each chamber and allowed to adhere before stamp removal, leaving separated cell patterns on the slide.
HUVEC seeded in serum-free media in 8-well chamber slides coated with complete Matrigel (4×104 cells/well) were allowed to adhere before being sandwiched with another layer of Matrigel that was uniformly pipetted on top of the adherent cells.
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After the League of Nations failed as an effective international body, Koht again favoured the strict neutrality policy to which Norway had adhered before the League of Nations membership.
However, there has been ongoing discussions and frequent stakeholder's consultations focussing on how to adapt the WHO's global recommendations which provide a recipe to be adhered before attempting to scale up task shifting.
In general: Plate 2.5 x 10 of the target cells (BaF3 in our case) in each well of a 6 well plate using fresh medium (if adherent cells are used they should be allowed to adhere overnight before infection).
Cells were plated in 6 well plates (105 cells/well) and allowed to adhere overnight before the addition of test compounds.
Cells were allowed to adhere overnight before treatment.
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CEO of Professional Science Editing for Scientists @ prosciediting.com