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It is imperative to employ Before After, Control-Impact monitoring designs with adequate replication, and multiple independent reference locations to assess potential impacts adequately.
Because of their resource-consuming nature, many facilities and studies are often lacking in adequate replication.
A key challenge facing scientists in marine ornamental aquaculture is the successful design and operation of tanks that accommodate fragile pelagic larvae, whilst allowing for adequate replication, behavioural observation and systematic investigation.
Additional approaches have been suggested when scientists are incapable of achieving adequate replication.
Oksanen [32] countered this argument by pointing out that ecologists must choose between sacrificing the appropriate spatial and temporal scale at which to conduct an experiment in order to achieve adequate replication vs. using the appropriate scale without adequate replication.
For data analysis, samples were pooled into "populations" representing all corals from sites within a region/depth to ensure adequate replication for statistical comparison and also because we have shown previously that DNA sequence does not resolve genetic structure among sites within geographic regions for deep sea corals [8].
More refined null expectations and adequate replication are needed to allow more rigorous conclusions.
These findings emphasize the importance of conducting large-scale studies with adequate replication.
By good experimental design we refer to the usual statistical rules-of-thumb of adequate replication and randomisation.
Numerous sources suggest five arrays per treatment group can provide adequate replication for such analyses [ 50, 51].
Thus, it is highly desirable to conduct large-scale studies with adequate replication to identify variants that are likely to exert an effect on life span.
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CEO of Professional Science Editing for Scientists @ prosciediting.com