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Determination of orexin in plasma is hampered by the lack of adequate assays, in terms of sensitivity and specificity.
While the quality of the predictions will remain high, the sensitivity will be diminished in datasets with less separation (due to lack of adequate assays or technical variability).
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The FDA draft guidance emphasizes the need for condom use also for vasectomized men unless measurements using an adequate assay fail to detect the molecule in seminal fluid.
Given these variations and factoring in the relatively narrow range of boundary iron concentrations used in this profile (the latter largely being determined by the need to maintain adequate assay signal), an overreliance on this filtering step to exclude series from further consideration carries the risk of losing viable scaffolds.
Determination of reaction efficiency was routinely used as an internal quality control for adequate assay performance.
It is thus important to monitor kidney function regularly, using an adequate assay.
However, so far no inhibitory effect on the full LP has been reported due to the lack of an adequate assay.
Although adequate screening assays are available nowadays, development of liquid chromatography tandem mass spectrometry (LC-MS/MS) confirmatory methods to detect low levels of rbST is still a challenge.
The developed method has shown precision, linearity, specificity, and limit of quantification (LOQ) adequate to assay fluoxetine and norfluoxetine in plasma.
When new targets are identified, there are usually no such small molecules known, which requires the use of high throughput screening (HTS) of a chemical library, if the adequate experimental assay exists, to find hits that may constitute good leads for drug design.
This question can be addressed with an adequate reporter assay, as described by others [12].
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CEO of Professional Science Editing for Scientists @ prosciediting.com