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To address whether the increased CD39 and CD73 enhanced the suppressive function of Treg cells, we used adenosine receptor A2A antagonist SCH-58261 for the in vitro suppression assay, since adenosine is the hydrolytic product of CD39 and CD73 and mediates the immunosuppression [13].
Our study did not address whether the increased Tor signaling is a cause or result of the energy metabolism of the buffy mutant.
Considering that mRNA levels do not always correlate with protein levels, further study may be necessary to address whether the increased mRNAs from metabolic genes are linked to the control of enzymatic activities.
To address whether the increased heart weight in offspring of obese dams was a consequence of cardiac remodelling, nonbiased cardiac stereology was performed to assess both ventricular wall and chamber volumes.
However, future studies should address whether the increased mortality during winter in temperate zones is amendable to intervention.
To address whether the increased levels of mtDNA observed in ref(2 P mutants were associated with enhanced heteroplasmy in mtDNA because of possible defects in mitophagy we used Next-Generation Sequencing (NGS) technology to sequence the mitochondrial genome in control and ref(2 P mutant flies.
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It was specifically addressed whether the increased ICP wave amplitudes seen in iNPH shunt responders were accompanied by elevated CO and/or ABP wave amplitude levels.
One question that has thus rarely been addressed is whether the increased risk of cancer is simply due to the lack of one healthy copy of the gene (haploinsufficiency) or whether the gene produces a protein product that interferes with normal cellular processes.
To address whether the increase in CCN2 expression in response to strain was dependent on TGFβ, we initially performed real-time PCR analysis on mRNAs treated with or without mechanical strain for 72 hours.
We then analyzed BrdU incorporation in the GFP+ cell population to address whether the increase in the number of pericytes is due to cell proliferation.
To address whether the increase in October 2009 reflected a clonal outbreak of 7F, we analyzed the proportion of IPD cases in Denver that were serotype 7F during 2004 2010.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com