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Prior to a definitive trial further work on composite outcomes is required to address whether the effect is maintained across different individual endpoints within the composite outcome.
To address whether the effect of DDT on VEGFA expression in MCF-7 cells is dependent on E2 or ERα, we assayed VEGFA expression by qPCR in MCF-7 cells incubated in the presence of the ERα inhibitor ICI.
To address whether the effect of CX3CL1 on monocyte apoptosis is specifically mediated via CX3CR1, we used a receptor antagonist (AZ12201182 compound 18a, referred to here as AZ), which inhibits CX3CL1 responses in multiple assays.
Future research should also address whether the effect found here generalizes to sounds varied across a range of dimensions for example, volume, pitch, and timbre while also considering the signal-to-noise ratio.
To address whether the effect of TNF antagonists (10 μg/ml each) on T-lymphocyte activation was restricted to the anti-PPD response, we tested their effect on the proliferative response against Cd, CMV, and TT.
DOI: http://dx.doi.org/10.7554/eLife.08939.011 We tried to address whether the effect of IREF-2 on vRNA and cRNA syntheses could be distinguished by using a mini-genome replicon system with either the vRNA reporter (vNS-Luc) or the cRNA reporter (cNS-Luc) as the source of the viral genome.
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The new studies, financed by the agricultural biotechnology industry and the government but carried out mainly by academic scientists, address whether the effects seen in the laboratory are also found in the field.
In order to address whether the effects of aldoaa and kif22 LOF were due directly to changes in expression within the brain, or secondarily, owing to effects on other tissues, shRNAs targeting these genes were expressed in the zebrafish brain.
To address whether the effects of PHY906 were tissue specific, we compared the transcriptional changes at the tumor site with those in two normal organs characterized by high metabolic activity (liver) or immunologic functions (spleen).
To directly address whether the effects of FOXA1 in promoting EC cell proliferation can be attributed to its activation of AR, a rescue experiment in MFE-296 cells was performed.
An approach to address whether the effects (and potential side-effects like metabolic dysfunction) of rapamycin in otherwise healthy rodents may also be relevant to humans is to perform such experiments in other species that are predicted to have similar phenotypic metabolic regulation as humans.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com