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We address this method as DFTDIT−DIF−Pr.
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To address this, methods have been developed using modified primers during the first strand of cDNA synthesis [ 29], however these methods are not suitable for application after library synthesis.
To what extent such overlap-correction is applicable is an important issue to address for this method.
There is a practical consideration about the consumption of the protein to address before this method can be applied in protein crystallization screens.
It is thus difficult to address, by this method, the precise functions of these highly homologous proteins.
One important question we wish to address using this method is the order of events leading to the formation of a pore.
To address this, our method does not require genetic observations at the same loci.
Species-specific genes are also not addressed using this method.
Seven of these had postal addresses, so this method was used in these instances.
The challenge of genetic heterogeneity has yet to be addressed with this method as well.
Broadening the perspective, we think other questions in applied medical ethics could be addressed using this method.
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