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Most studies, including ours, address the expression patterns of specific miRNAs rather than a more general process involving miRNA expression.
We now address the expression patterns targeted by the GAL4 lines in the last Discussion section that also covers comment 5 from this reviewer (last three paragraphs of the discussion).
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In order to address the expression pattern of CDC-42 at later developmental stages, we created a transgene containing the genomic cdc-42 coding sequence under its endogenous 5′ and 3′ regulatory sequences fused N-terminally to GFP (opIs295[P cdc-42 ::gfp::cdc-42 3′ cdc-42 3′ ] FigUTR 3a).
Next, we addressed the expression patterns of sox2, tlx, gli3, and her9 with respect to their putative target gene rx2 in the juvenile CMZ by two-color fluorescent whole-mount in situ hybridization (WISH).
We have also addressed the expression pattern of CEACAM6 in primary tumors and in matched metastases in the same patients.
Therefore, we addressed the expression pattern of C. arietinum Ca1_5 and M. truncatula Mt2_10, Mt2_11, and Mt7_7 genes.
To address these questions, we analyzed the expression patterns of transcriptional reporters that express 4xNLS::GFP under the control of the three C. elegans DNase II gene promoters.
To address this possibility, we analyzed the expression patterns of the 1,118 LUATs and their associated genes found in the multi-tissue analysis.
To address the need for studying the expression patterns of hordein genes, a rapid qRT-PCR screening method was developed and verified.
In order to address this question, we examined the expression patterns of peach homologues of Arabidopsis genes known to control dehiscence.
To begin to address this hypothesis, we determined the expression patterns of seventeen conserved meiosis-related genes in the N. vitripennis testis and ovary (Table S11); these genes are the previously predicted orthologs of meiosis-related genes in D. melanogaster (FLYBASE: FB2015_03).
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CEO of Professional Science Editing for Scientists @ prosciediting.com