To further address origin firing, we investigated the association of the replication initiation factor Cdc45 with the rARS in WT and eco1 mutant cells using ChIP 29, 30.
To address origin occupancy by the initiator proteins in vivo, we performed chromatin immunoprecipitation using antisera generated against Orc1-1, Orc1-2, and Orc1-3 and WhiP.
In keeping with the objective of this article, we chose to focus on the students' responses to questions that addressed origin of variation because these questions specifically probed for understanding that "random mutations and sexual reproduction produce variations…" (Anderson et al., 2002, p. 965).
And, two, that the charophyte green algal transcriptoms are remarkably plant-like, which gives them the unique capacity to be major players for future evolutionary genomic studies addressing origin of land plant questions.
Here, we address the origins of multimap tags and present a novel strategy to assign CAGE tags to their most likely source promoter region.
Several hypotheses address its origins, but none adequately explained at the genetic level how this organelle might have arisen.
Chapters address the origins of email encryption and why email encryption is rarely used despite the myriad of its benefits -- benefits that cannot be obtained in any other way.
In the following, we will address the origins of the above-described carbonaceous signatures from both abiotic and a biogenic points of view.
Students commonly have misconceptions about how mutations affect phenotype and questions that address the origins of variation are challenging for them to answer correctly (Gregory, 2009).
To address the origins of the MCRA phenotype, we have determined the spectrum of somatic mutations at APC, K-ras and BRAF in tumours from 25 MCRA patients with no detectable germline mutation in APC, MYH or the MMR genes.
