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To address origin occupancy by the initiator proteins in vivo, we performed chromatin immunoprecipitation using antisera generated against Orc1-1, Orc1-2, and Orc1-3 and WhiP.
To further address origin firing, we investigated the association of the replication initiation factor Cdc45 with the rARS in WT and eco1 mutant cells using ChIP 29, 30.
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Indeed, " Why genes-in-pieces" did not address origins, or seek to explain the presence of spliceosomal introns in (all) eukaryotic nuclear genomes and their absence from (any) prokaryotic genomes.
In keeping with the objective of this article, we chose to focus on the students' responses to questions that addressed origin of variation because these questions specifically probed for understanding that "random mutations and sexual reproduction produce variations…" (Anderson et al., 2002, p. 965).
And, two, that the charophyte green algal transcriptoms are remarkably plant-like, which gives them the unique capacity to be major players for future evolutionary genomic studies addressing origin of land plant questions.
Here, I do not address the origin of the heat source and simply assume the existence of a molten layer of thickness D inside an icy object.
We also address the origin of the Ainu lineage in northern Japan.
Although from the epistemological perspective Rice et al.'s assertions may be valid (i.e., Darwin did not address the origin of life, nor of the universe, in The Origin of Species of 1859, but see below), from the empirical reality viewpoint the origin of life is inconceivable without a prior evolutionary process or explanation.
Fourth, the current article does not even attempt to address the origin of the ribosome whereas to me any discussion of the origin of translation that does not consider ribosomes is woefully incomplete.
With the synonymic underbrush thus cleared, we can now address the origin of yee-haw!
All 60 of these donations listed the Kushner Companies headquarters as the address of origin, and each one of the contributions to Mr. McGreevey was for exactly $2,000.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com