The original PDB entries and the optimized structure models were additionally validated with MolProbity (Davis et al., 2007).
The PDB files are additionally validated with PDB-care (Lutteke and von der Lieth, 2004) to detect superfluous carbohydrate atoms.
The conservative phase follows the original PDB_REDO method (Joosten et al., 2009) with several enhancements: The PDB files are additionally validated with PDB-care (Lutteke and von der Lieth, 2004 ) to detect superfluous carbohydrate atoms.
The upregulation of MMP9 and IGF1 was additionally validated with qPCR (data not shown).
Interaction interfaces in complexes are additionally validated using the PISA algorithm.
We have now additionally validated the phospho-specificity of this antibody in the C. elegans germline, by showing that staining disappears in germlines treated with lambda protein phosphatase.
Next we additionally validated the role of Sphk-1 in NO in mouse primary macrophages.
We additionally validated the plasma C/D ratio as a prognostic value in 96 ESCC patients.
Open reading frames attributed to heavy metal resistance were additionally validated by sequence comparison with NCBI-NR using BLAST program.
Our findings were additionally validated by Sanger sequencing of the respective genomic loci.
