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The technology utilizes cells derived from the cochlea of mice.

Additionally, utilizing flow cytometry (FCM) techniques to identify B cell populations, we analyzed PIP2 levels and calcium mobilization in transitional and mature B cell subsets, again using as few as 1x10 total B cells.

Additionally, utilizing flow cytometric techniques, we determined levels of phosphatidylinositol bisphosphate and calcium mobilization in T1 and T2 cells, as well as mature follicular and marginal zone B cells using less than 1x106 primary B cells.

We additionally utilized the MCF-7 cell line, a classical breast cancer (BCa) established from the metastasis (pleural effusion) of a human breast adenocarcinoma.

Additionally, variability between cells from different donors introduces a complicating factor.

Additionally, cells from the different skin layers also stained positive.

Additionally, success relies on utilizing a cell source that is unaffected by pathology and is feasible for surgeons to isolate.

The FLAG epitope tagged Kv3.1 cDNA molecules were acquired from previous study utilizing Sf9 cells [5].

To this end we utilized 728 cells, a mouse squamous cell line derived from an EphA1/EphA2 double knockout mouse (see Methods).

Combined information was utilized to discriminate tumor cells from WBCs.

Single cell-derived clones were isolated utilizing fluorescence-activated cell sorting from genetically engineered 231-BrM2 (WT10, MUT3) and CN34-BrM2 (WT6) cells.

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