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The complete list of 427 genes in Group 2 can be found in Additional file 2. These genes were additionally submitted to manual inspection and comparison to gene data from the free-living non-parasite platyhelminth S. mediterranea (a planarian).
All sequences were additionally submitted to the Phyre protein fold recognition server.
Sequences with discordant results or for which the subtype remained unassigned by at least one of the two tools were additionally submitted to SCUEAL and jpHMM.
Cases scored as 2+ were additionally submitted to chromogenic "in situ" hybridization (CISH, Zymed's SpoT-LightR HER2CISH™, Invitrogen, San Francisco, CA, USA), according to the manufacturer's instructions [ 23].
Tissues from 9 fish were additionally submitted to Aquatic Diagnostic Services at the Atlantic Veterinary College in Prince Edward Island, Canada, for histological evaluation.
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Additionally, it was submitted to and approved by the Research Alignment Committee of the Santo Antônio Children's Hospital of Porto Alegre under number 1.234.760 (CAAE: 46701115.3.1001.5683) as part of the data collection which was done at this institution.
Additionally, the animals submitted to pneumosepsis had a loss of aversive memory 13 days after pneumonia induction and this loss remained 50 days later.
Additionally, all purchases are submitted to a database with documents demonstrating that medicines are of good quality.
Additionally, alignments were only submitted to further analysis, if the second best alignment resulted in an e-value that was at least 10-fold larger than the e-value of the best alignment.
Hospitals entered colposcopy details into a study database and additionally submitted anonymised pathology reports to Barts Health NHS Trust.
Additionally, you can view photographs submitted to The Times from people who live in and among highrises around the world.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com