Sentence examples for additionally stained with from inspiring English sources

Tumour slices were additionally stained with CD-31 antibody to quantify microvessel density (MVD).

For optical assessment by flow cytometry, cells were additionally stained with CD14 and CD16.

The spinal cord was additionally stained with Luxol fast blue.

PBMCs were additionally stained with anti-CD3 and anti-CD14 antibodies.

As a reference in each tissue, the nuclei were additionally stained with haemalum.

As a reference within each tissue, the nuclei were additionally stained with haemalum.

Additionally, staining with a GPR110 antibody enabled us to differentiate between benign prostate hyperplasia and potential incipient malignancy.

Samples were additionally stained for DNA with Hoechst 33258 (0.5 µg/ml in PBS, Invitrogen), for 15 min and mounted on slides with 12 mm-round coverslips.

To compare the postembryonic part of the clones to the ones described previously (Truman et al., 2004), we additionally stained all specimens with an antibody against Neurotactin (Nrt; Fig. 1C″), which is exclusively expressed on the surface of immature neurons (de la Escalera et al., 1990) and serves as another criterion for secondary neurons.

For MSC morphology studies, MSCs were blocked with 1%% bovine serum albumin and additionally stained for 1 h with Alexa Fluor 547 Phalloidin (1 3,000; Life Technologies) and fluorescein isothiocyanate (FITC -conjugated α-tubulin (1:1000; Sigma).

After fixing the cells with 4% paraformaldehyde (PFA) for 25 min at room temperature, nuclei were additionally stained for 5 min with 1  μg/ml DAPI (Sigma) in PBS supplemented with Ca2+ and Mg2+ (PBSD+).