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Then, the medium was exchanged into 250 µL of DMEM and yeasts were additionally cultivated for 12 h at 30°C.
Additionally, cultivated area and fertilizer (types and amount) distributed per year of in the study districts were collected from secondary sources.
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Additionally, cultivating friendships with only a select number of staffers can create feelings of distrust and resentment.
Additionally, we cultivated E. coli BL21 (DE _pET20b _pepP- Lc in shaking flasks at 37°C and cooled the cultures to 4°C and 20°C prior induction.
Additionally, mushrooms are commonly cultivated on single substrates, commonly wheat or paddy straw (Ragunathan and Swaminathan 2003).
Additionally, it has been cultivated from flowers and fruits using highly selective culturing methods (Endo et al. 2012; Neveling et al. 2012; Anderson et al. 2013).
Additionally, the recombinant strain was also cultivated in the absence of L-malic acid.
In order to address the problem, the metabolome data was additionally obtained from the laboratory yeast strain (S288C) cultivated with l-methionine (S288C Met in Figs. 1, 2).
Additionally, a non-transformed K. lactis wild type was cultivated as a reference.
SR additionally provided the samples for the Synechocystis, which were cultivated, processed and measured on the mass spectrometers by NP.
The cell were cultivated for the next 20 hours, stained additionally with antibody against gelsolin (as above) and then observed by fluorescence microscopy.
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