The analytical procedure additionally contained a correction for incomplete hemolymph equilibration at the lowest PCO2 step (Additional file 3).
As wash- and binding buffer, 50 mM NaPP buffer (300 mM NaCl, 0.01% TWEEN® 20, pH 8) was used, while the elution buffer additionally contained 300 mM imidazole.
Cells were cultured in MCGC minimal medium containing 25 μg/mL kanamycin, and the medium at the panel (A) additionally contained 2 mM H2O2.
Extracts used for antioxidant enzyme assays were prepared using 50 mM potassium phosphate buffer (pH 7.0) containing 1 mM EDTA-2Na, while buffers used in TBARS and GSH and GSSG assays additionally contained TCA (100 and 50 mg/mL, respectively).
When using synthetic miRNA (Fig. S4) the transfection solution additionally contained 1 pmole of duplex miRNA.
For R1 cells, differentiation media additionally contained 1 mM sodium pyruvate.
Assay plates for discrimination assays additionally contained 50 mM NaAc, pH = 6.0 or 100 mM NH4Cl, pH = 6.0.
When the oxidation was performed in the presence of ascorbate, the incubation mixture additionally contained 40 µM of ascorbate.
In the case of those chimerical enzymes that distributed mainly to the insoluble fraction the lysis buffer additionally contained 300 mM NaCl.
The PA− and PA+ foods were identical to the standard rearing diet [49], except the PA+ food additionally contained 0.1% monocrotaline, a representative PA.
The second construct additionally contained four miR-29 MREs, and was significantly responsive to both MREsandrecursors, indicating a specific effect of miR-15 and miR-29 MREs in the CDS of murine Eln.
