Induction of autophagy in oxidatively stressed TM cells was additionally confirmed using a commercially available kit recently launched to the market (Cyto-ID® Autophagy Detection Kit, Enzo Life Science), which allows quantification of autophagy by flow cytometry.
Anti-PA28γ autoantibodies were additionally confirmed using a newly developed microbead assay.
This issue was additionally confirmed using only CZE mode, to avoid additional equilibria due to MEKC mode (Fig. 2).
Additionally, we confirmed using ISOI that protection was granted not only to the stimulated whisker but also to other ipsi-ischemic whiskers.
Additionally, Pi2 was confirmed using two flanking markers, RM527 and HC28 in the MF4 (Jiang et al. 2012; Jiang et al. 2012).
Additionally its existence was immunohistochemically confirmed using a panel of cytokeratins (CK8/18, CK5/6 and CK14).
Additionally, alternating structures of the copolymers were confirmed using 13C NMR, which reveals triad distributions.
Expression of zeta isoform of aPKC (aPKCζ) was confirmed using antibody labeling, which additionally revealed that its subcellular localization mirrored the distribution of MARCKS in 2M ECs, enriched at the apical surface (Fig. 1B, Movie S3).
Additionally, the reaction mechanism of probe 1 and hydrogen peroxide was confirmed using 1H-NMR and ESI-MS.
Additionally, the presence of Hv1 homodimers in all major types of human leukocytes and in differentiated PLB-985 granulocytes could be confirmed using DSS (Fig. S2b).
