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The four suppressor mutants additionally carried non-synonymous SNPs in fliM encoding a flagellar switch protein.
To get more detailed information about short term variations of flow and recharge behaviour of the karst system, high resolution monitoring of discharge, EC and T was additionally carried out for 1 month during rainy season (February 2012) in one of the focused underground rivers called Seropan River which flows through Gua Seropan (Gua is Indonesian for cave).
In order to remove residual lignin and carried out partial hydrolysis of hemicellulose, we additionally carried out thermochemical treatment of OSP using acetic acid and hydrogen peroxide in a volume ratio of 70 30% with the catalyst sulfuric acid which was 15% to a.d.r.m mass.
We first performed conventional PCR amplification using the wsp gene of Wolbachia [42], and then additionally carried out high-resolution quantitative PCR of the ftsZ gene (Roche 2.0 LightCycler and SYBR green; Roche Biochemicals; [48]) for samples for which no infection could be scored.
Because of the difficulty to discriminate between C. psittaci and C. abortus and not possible on the basis of the major outer membrane protein A, we additionally carried out a. MLST analyses as described by Pannekoek et al. http://www.pubmlst.org/chlamydiales).org/chlamydiales
AW additionally carried out microarray analysis.
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More advanced knockout constructs additionally carry loxP sites on both sides of the Bsr cassettes for subsequent removal of the selection marker by transient expression of Cre recombinase, thus allowing generation of multiple knockouts using just a single selection marker.
Corollary 35 considers systems which additionally carry a twisted reflection symmetry (or equivalently have no imaginary hopping terms) as discussed in [19].
Additionally, carrying a weapon slows Faith down; the heavier the gun, the more it hinders her movement.
There is a duplex along the highway where it additionally carries the B72 route marker between the two sections of the Snowy Mountains Highway.
The AML1 N-terminus additionally carries transcriptional activation domain activity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com