For our blood pressure and LDL analysis, we additionally ascertained antihypertensive medication use and statin use, respectively.
Sample extracts are then transferred to new nuclease-free 0.2 ml microvials (MidWest Scientific) and the desired final volume of 36 μl for each sample is additionally ascertained during this final sample transfer (or samples are used right away in the real-time RT-PCR application and not transferred).
Additionally, we ascertained current practice with respect to Ureaplasma detection in preterm neonates in the UK.
Additionally, we ascertained genes expressed primarily in the sex tissues of males (testis and accessory gland) or females (ovary) by calculating breadths of gene expression (see Materials and Methods for details).
Additionally, to ascertain whether these stress-responsive genes were involved in the response to cold or drought stress in A. mongolicus, we compared these stress-responsive genes with previously reported abiotic stress genes from other organisms documented in the Comparative Stress Genes Catalog of GCP (Generation Challenge Programme, http://www.generationcp.org).
Additionally, to ascertain cell death, cells were incubated with 2.5 mg/mL propidium iodide (PI) for 5 min at room temperature and analyzed with a FACSCanto II flow cytometer (Becton Dickson, Franklin Lakes, NJ, USA) and Flowing Software (version 2.5.1; http://www.flowingsoftware.com/).com/
High-magnification and low-magnification (100× and 20×) DIC images were additionally recorded to ascertain the position of sperm cells.
Additionally, it is ascertained that the hydrothermal synthesis method is able to produce a spherical shaped submicron particle.
Additionally, and to ascertain the robustness of the findings, the three specifications above are also estimated with two different control variables and an alternative indicator of trade openness.
Additionally, frailty was ascertained prior to the report of falls, so its predictive value could be assessed.
Spearman's non-parametric correlation tests were additionally carried out to ascertain correlations between age and miRNA expression levels.
