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The dataset was additionally analyzed with the machine-learning algorithm RF in order to address issues of model overfitting and markers were selected based on their variable importance ranking.

Aliquots of all cervix cancer samples were additionally analyzed with the ELISA system previously marketed by this manufacturer under the same name which was used in previous publications on OPN as an endogenous hypoxia marker [ 1, 2].

All head and neck cancer samples were additionally analyzed with the current IBL assay which should be identical with the old TiterZyme assay and was therefore designated "assay B2".

All five pathology groups were additionally analyzed with one-way ANOVA tests to determine which of the three ultrasonic parameters provided statistically significant separation of all five groups.

Transcripts with a significant time*treatment interaction (p < 0.05) were additionally analyzed with a one-way ANOVA at each time point with treatment as the independent variable (p < 0.05).

To verify speciation, all Gram-negative rods tested with API 20E were additionally analyzed with PCR with species-specific primers for E. coli[ 20].

A set of 17 selected genes from several differentially expressed pathways was additionally analyzed with quantitative real-time PCR and confirmed to be suitable for a reliable classification of infected plants and for the characterization of susceptibility features in the field conditions.

Clinical characteristics, including age, sex, tumor location, and TNM stage, were additionally analyzed for the association study with GSTT2 genotyping.

The relationships among cyt b haplotypes were additionally analyzed and visualized with the statistical parsimony method implemented in TCS 1.21 [ 70].

To compensate for the uncertainty caused by missing values from drop-out individuals, data were additionally analyzed using the multiple imputation technique with 10 replacements at random in order [ 21] based on the confirmation that the missing pattern was completely at random [ 22]; these results are presented in brackets.

Seven cats with platelet aggregates were additionally analyzed on the Mythic 18 instrument (Orphée SA) for total WBC counts at two time points [ 9].