Exact(3)
By using this additional spindle detection we assign physiological meaning to a separate spindle process, which, while being correlated with stage S2, is still independent from stages.
During mitosis in living WT cells, k-fibres appear to be rapidly reinforced by the formation of additional spindle MTs, a process that appears to be lacking in Dgp71WD, Augmin and γ-TuRC mutant cells.
Similar results have been obtained in yeasts, where it is possible to drive the production of additional spindle pole bodies through mutation (Flory et al., 2002; McGrew et al., 1992; Shirk et al., 2011), and these arrangements lead to aberrant spindles and DNA mis-segregation.
Similar(57)
An additional checkpoint, the spindle assembly checkpoint, monitors the proper alignment of chromosomes during mitosis and can be activated by anti-mitotic drugs such as paclitaxel.
Additional PPIs involving spindle checkpoint proteins were gathered from several curated PPI databases.
Taken together, ASPM may have additional function besides spindle positioning and division axis orientation critical for symmetric cell division.
Cells treated with either GF 10 μM or vinblastine 0.5 nM exhibited nearly normal bipolar mitotic spindles (Additional file 1, Figure S6).
Background EEG was included in four test signals, modeled either as small additional sinusoids across the spindle frequency range or as filtered Gaussian noise segments.
It can be seen that most of the milled CL-20 microparticles form sphere shape with smooth surface after ball milling, whereas the starting material presents spindle shape (Additional file 1: Figure S2c).
The combined treatment of GF and vinblastine strongly disrupted the organization of the spindle microtubules (Additional file 1, Figure S6).
This suggests that Bub1-cm1 and the Mad1 C-terminus have an additional role within the spindle assembly checkpoint, apart from recruiting Mad1 and Mad2 to kinetochores.
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