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P53 pathway-focused expression profiling using RT-PCR arrays confirmed the response of many p53 targets and also identified additional responsive p53 targets in all three treatment groups that were not found by microarray analysis (Table I).
"N.Y.U. is continuing to collect and process additional responsive materials," the letter said.
Three trials undertook additional responsive PPI activity that had not been specified in their documented plans.
To identify additional responsive genes, we transferred rice seedlings at the six-leaf stage to an N-deficient hydroponic solution.
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Here, we report the discovery of six additional mtUPR responsive genes.
However, starvation of animals may activate additional stress responsive transcription factors such as heat shock factor (HSF1).
Since these arrays do not contain miRNAs, we were not able to detect additional p53 responsive miRNA clusters.
Additional copper responsive oxidative defense enzymes detected by whole cell proteomics analysis includes a plasmid encoded DSBA oxidoreductase (Krad4572), a member of the thioredoxin family that catalyzes disulfide bonding and proper folding of secreted proteins.
Instead we demonstrate that Pho7 binds within the promoters of additional stress responsive genes and plays a role in iron, copper, osmotic, and alternative carbon utilization stress.
Further analysis of the RNA-Seq and cDNA data for sequences not represented on the array revealed additional genes responsive to PCB (induced or repressed) in both populations.
Such additional CEBPA responsive promoter elements were reported for CEBPA regulation of miR-223 (Fazi et al, 2005; Eyholzer et al, 2009) or miR-661 (Reddy et al, 2009).
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