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In addition to the mature miRNA and the miRNA star, additional reads are present for some intervals.
These additional reads are generally ignored, potentially wasting an important source of genomic data.
An update involves multiple disk reads and writes to the header and archives within the database and if consolidation occurs there are additional reads and writes.
Alternatively, additional reads can be produced by simply sequencing a sample multiple times.
For the hyper-variable V6 region, over 45 additional reads are needed for each new phylotype (>97.8% coverage).
Additional reads matching at least 12 out of any 13 consecutive adaptor nucleotides were excluded from the analyses.
Regardless of the simulated average coverage, they were the outcome of random errors in sequencing that could not be corrected by additional reads covering the same position.
The higher threshold gives 9 additional reads.
Second, we consider the effect of additional reads on enrichment score, saturation, and coverage.
Swift produced significantly more reads than the GAPipeline (∼ 10 additional reads).
Repeated sequencing of this library resulted in 22 additional reads of orthobunyavirus-specific sequences.
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