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He recorded several additional "poppy" vocals of his parents—none of which were used on Syro and stored "entire sample packs of their voices" during the process.
Two additional poppy proteins [Uniprot:Q4QTI9], [Uniprot:Q4QTJ0] as well as a protein from Coptis japonica [Uniprot A2A1A1], a plant closely related to T. flavum, have been designated PR-10 but showed close similarities with NCS.
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Additional reporting by Saw Nang Poppy McPherson is a journalist based in Southeast Asia.
An absolute quantification or information on relative abundance (i.e. either an absolute cellular concentration, or a reliable determination that the cellular pool size was below the analytical detection limit) was obtained for 144 cellular metabolites among a total of 212 diverse compounds in the customized opium poppy database [see Additional file 2].
For the purposes of this study, one-dimensional H NMR signatures corresponding to selected compounds not present in the standard Chenomx library, including those of several benzylisoquinoline alkaloids, were used to create a custom opium poppy database [see Additional file 2].
*After removal of sub-standard sequence **Sum of contigs and singletons ***BLASTx search of the UniProt Plants v14.8 database (e-value cutoff of 10-5) The discovery rate of new unigenes reach saturation after approximately 250,000 pyrosequencing reads suggesting that near-complete representation of the elicitor-treated opium poppy cell culture transcriptome was achieved (Additional File 3A).
In addition, six P. bracteatum unigenes with substantial homology (up to 92 % amino acid identity) to opium poppy salutaridine reductase (SalR) were identified (Fig. 2, Additional file 14).
Moreover, the saturation of newly discovered unigenes after the analysis of approximately 250,000 ESTs (Additional File 2A) suggests near-complete coverage of the elicitor-treated opium poppy cell culture transcriptome.
Interpretation of positive opiate screens is complicated given the complexity of opiate metabolism and multiple possible sources of opiates such as medications prescribed for the mother and/or newborn, poppy seeds, heroin, or intentional misuse of prescription opiates (See Additional file 1) [ 9].
An additional 5,000 American troops would probably be sent to the contested Helmand Province, home to the poppy crop that is a major source of income for the Taliban who traffic in opium.
A customized opium poppy NMR spectral library was created to identify and quantify individual metabolites [see Additional file 2].
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