As described in previous study [ 14], the additional opposite-arm miRNAs are not necessary to be at lower expression levels than the original ones.
With the improvement on NGS's sequencing intensity, we detected additional opposite-arm mature miRNAs at the pre-miRNAs originally encoding only one miRNA.
In this study, we totally detected 24 additional opposite-arm miRNAs exclusively in G1245N, 33 ones exclusively in G1245T and 63 ones both in G1245N and G1245T library.
The discovery of additional opposite-arm miRNAs is because we mapped the sequence reads back to pre-miRNAs rather than only to mature miRNAs.
Many of the additional opposite-arm miRNAs have higher expression level than the original one (Additional file 2 and 3), which might be different from the previous nomenclature rules.
The induced currents are implemented in the model by including an additional term (with opposite sign and a different radius of the currents) in the design matrix elements, (g_{n,k}), presented in Eq. 3.
Statistical testing also provided support for two additional QTLs of opposite signs, one conferring HW-like behavior (QTL4) and one conferring N2-like behavior (QTL6).
Our across-strain analysis identified two additional QTL with opposite effects: QTL3-2 and QTL5-1 inumbered number of fruit but decreased average fruit weight (Table 4).
However, whereas Valverde et al. [ 89] detected gap3 transcripts in heterocysts and vegetative cells of A. variabilis, but none of gap1, we found the opposite (Additional file 7).
When also considering K point depression, additional sensitivity in opposite segments, particularly in the midbasal layers, resulted in a less distinct pattern (compare, e.g., V6).
Moreover, the favored haplotype combination A/1 occurred at a higher frequency in the modern cultivars than in landraces, whereas small grained G/4 was the opposite (Additional file 8: Figure S7).
