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They expanded their investigation and developed models applicable to lizard species at more than 1,200 additional localities in South America, Africa, Europe, and Australia.
The additional localities were from the literature [45] [49] or from specimens at the California Academy of Sciences.
Individuals were collected between September 2005 and March 2009 throughout the European Alps France, Italy, Switzerland, Germany, Austriaa, Slovenia) and from additional localities in the Pyrenees and the Vosges Mountains Francee), the Jura Mountains (France, Switzerland), the Tatra Mountains (Slovakia, Poland, Hungary), and the Bohemian Forest and the Sudete Mountains Czech Republicc; Fig. 1).
We also included ND4 and cytochrome b sequences from GenBank for 43 salamanders from 27 additional localities [ 35].
That SAFV-2 can persist between years suggests that it might be common, yet underreported, in Denmark, which provides the opportunity for spread to additional localities.
Samples for seven additional localities in Canada and Alaska were sent to us by remote collectors or obtained from the stonefly collection at the Monte L. Bean Life Science Museum at Brigham Young University.
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Each transect was found on a distinct mountain ridge: Heintooga Ridge (HR) on Balsam Mountain, Kephart Prong (KP) on Richland Mountain, and Taywa Creek (TC) on Hughes Ridge (for additional locality information see [ 39, 51, 52]).
verna and potentially involved in the genesis of P. alpicola were sampled from additional six localities.
No additional sympatric localities between these transects have been found, and because Plethodon have low dispersal abilities [ 63], with 300 m being the maximal dispersal distance ever recorded [ 64], dispersal between sympatric localities is improbable.
However, the low sample numbers in the south likely indicate genuine scarcity in this region, and the discovery of additional southern localities would likely expand, rather than contract, the projected climatic distribution of this species.
However, nuclear gene tree analyses suggested little to no signal of geographic population structure within species as haplotypes were shared among many localities (Additional file 5: Figure S2, Additional file 6: Figure S3, Additional file 7: Figure S4).
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