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In addition, late in our study we obtained an additional isolate from P1 which was isolated 90 months after P1E (and which we designated P190) and a second sputum isolate from P2 isolated 64 months after P1E.
In 0.6% pyrazine suspensions, a log-4 reduction was observed for one additional isolate that was not affected by the lower concentration.
An additional isolate, BACI051 is clearly derived from Dugway, yet two variants have accumulated additional mutations in sigH (spo0H), hpr/scoC, and ebrB.
Long branches leading to clade III and to the Hungarian clone (H7) (Figure 1) might point to particularly rapid rates of evolution or long periods of isolation, but these long branches might also be sampling artifacts that could be broken up with additional isolate sampling or polymorphism discovery (i.e. any of the branches connected to H1 could join to these two long branches).
Details of the 15 Costa Rican isolates are provided in Table 4. Information on the other isolates used in the analysis are given in Yuan et al. [16], except for LIQ0090, an additional isolate that was extracted from Liquidambar styraciflua in Riverside, Ca in 2009, CVC0145, alias ICPB 50031 [5], and COF0238, alias Funde-2 [26].
*One additional isolate was recovered from a penial exudate.
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The horizontal repository portion has a slight upward tilt that provides additional isolation isolating any mechanisms that could move radioactive constituents upward.
To confirm our results that elg1 fails to provide addition benefit to ctf7 smcellsells, we analyzed four additional isolates from an independent cross.
The second cluster is composed primarily of isolates from California, in addition to group 1 Upper Texas Gulf Coast isolates and 3 additional isolates from Colorado, Connecticut, and Illinois.
Four additional isolates of F. graminearum and two additional isolates of F. culmorum were examined for production of these mycotoxins at the optimal temperatures.
qPCR analysis of these additional late isolate also confirmed the two genes BCAM0280 and BCAM0276 were up-regulated over time in these two late additional isolates (Supplementary Table S6).
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