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The advantages of using of SVF include the avoidance of an additional culture period, a reduced risk of extensive cell contamination, and cost-effectiveness.
However, the resulting cellular sheets and strings shrank considerably and did not retain their shapes during additional culture periods for myogenic differentiation.
We found that 2 μm gratings increase the rate of neural differentiation, and that an additional culture period of 2 μm gratings in the absence of neurotrophic signals can improve the neural differentiation of hPSCs.
Park et al. produced chondrocyte/ECM membranes in high density culture, peeled the membrane from the culture dishes, consolidated them by centrifuge, and then generated an engineered cartilage construct by an additional culture period (Park et al. 2006).
Additional culture: NZFS 171: multiple spore isolate from fruitbody on decayed log (probably N. solandri var. cliffortioides), Whakapapaiti track, Tongariro National Park, 13 June 1984, M. MacKenzie (Lat. 39°13′S Long. 175°31′E; parent fruitbody, NZFRI-M 2069, no longer exists), GenBank KR063257.
Three additional culture conditions were utilized as described below.
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Additional cultures were exposed to 500 nM -nicotine with or without the addition of 50 mM ethanol, 100 nM methyllycaconitine (an α7∗-bearing nAChR antagonist), or both.
Using an evidence-based treatment protocol originally designed for Western HIV-positive patients, we developed an 11-step Chinese Life-Steps program with an additional culture-specific intervention option.
Medical records were followed up for all children with contaminated culture results who had 1 or more additional cultures within 7 days of the original culture.
The strains were sourced from different yeast culture collections, such as the Oenology Culture Collection of the Department of Viticulture and Oenology, University of California, Davis, USA and the National Research Institute of Brewing (NRIB), Japan; the additional cultures were acquired from the market.
Additional cultures were also exposed to this regimen and were co-incubated with BD1047 (100 μM), a specific inhibitor of ER-bound sigma-1 receptors, for the 24 h NMDA exposure.
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