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An additional control was provided by BSA coated wells incubated with serum of infected mice.
Details of pyrosequencing conditions, including optimization of PCR amplification using 0, 50 and 100% methylation controls are provided in Additional files.
A complete list of miRNAs sequenced in each library, normalized read counts, and fold changes between centenarians and controls is provided in Additional file 2: Table S2.
Additional geometric control is provided by using a reference grid, while solution adaptivity is achieved by using weights.
Additional transcriptional control is provided by cis-acting regulatory regions marked by H3K4me1 and H3K27ac [ 4, 5, 8, 9].
Additional postoperative pain control was provided for all patients using patient controlled analgesia pumps (PCA) loaded with morphine.
Additional controls were splenocytes stimulated with ConA.
Additional controls are Menu, ProgressBar and OpenFileDialog.
Moreover, ant colonies provided access to supplemental carbohydrates responded more aggressively to surrogate herbivores than did control colonies (to which no additional resources were provided) or colonies provided protein.
Additional loans were provided by the California Academy of Sciences and American Museum of Natural History.
Additional control samples from the Netherlands were provided by R.A. Ophoff.
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