Sentence examples for additional 2 hours from inspiring English sources

The mixture was stirred for additional 2 hours.

The plates are incubated with gentle rocking for an additional 2 hours.

The plate was re-sealed and shaken for an additional 2 hours before recording the OD600 again ("post-infection" measurements).

Adult mice were perfused-fixed in 4% paraformaldehyde, then fixed for an additional 2 hours in 4% paraformaldehyde.

For this, cells were treated with DMSO or 23dMB-PP1 for 1 hour, after which taxol/MG132 was added for an additional 2 hours.

Cells were then harvested, repeatedly washed, transferred to asparagine broth medium without glucose, and grown for additional 2 hours to induce LAC1 gene expression [79].

Lysates were mixed with 5 10 µg primary antibody overnight at 4°C, then with 50 µL Protein A-agarose suspension for an additional 2 hours.

Protein-antibody complexes were precipitated with 40 µl of protein-G-agarose (Pierce) for an additional 2 hours at room temperature.

One hour after infection, cells were loaded with CCF4-AM dye and incubated for an additional 2 hours at room temperature.

After 2 hours of pre-incubation at 37°C the cells were incubated for an additional 2 hours in the presence of various concentrations of the indicated inhibitors in a shaking incubator.

For quantification of intracellular bacteria, cells were washed and incubated with 150 µg/ml gentamicin (Sigma, Poole, UK) for an additional 2 hours before proceeding with cell lysis and plating.