Sentence examples for additional 2 days from inspiring English sources

Plates were rotated by 90°, grown in long-day for an additional 2 days, and then scanned.

On Day 5, early blastocysts were cultured for additional 2 days in a blastocyst optimum culture medium, which is G2.3 medium supplemented with 2000 U/ml of human recombinant leukemia inhibitory factor (hLIF; Chemicon, Temecula, CA, USA) and 10 ng/ml of human basic fibroblast growth factor (bFGF; Vitrolife).

Effector T cells were generated by 3-day stimulation with plate-bound anti-CD3/CD28 and then cultured for an additional 2 days in rhIL-2 (50 U/ml).

Cells were then treated with IWP-2 for 4 days, incubated for an additional 2 days in serum free differentiation media, then supplemented with insulin at day 9. Human ES and IMP cells were differentiation to the kidney lineage as previously described (Taguchi et al., 2014).

For analysis of p53, N-Myc, and Wig-1 expression after stress, IMR-32 cells were transfected with 40 nM siControl 1 day post plating, treated with 0.4  μM Irn, kept in culture for an additional 2 days, and then harvested for western blotting as described below.

Then quetiapine was administrated for an additional 2 days.

Meninges were cultured for an additional 10 days with a change of medium every alternate day.

Agents and vehicle were added on day 2 and growth continued for an additional 5 days.

Plants were incubated at 22 °C (day) and 18 °C (night) with 60 65% humidity for additional 18 days.

The decree lasts 90 days, but can be extended an additional 180 days.

His extension was extended for an additional 45 days on 13 March.