Exact(3)
In addition to template, PCR reaction mixes contained 1 × PCR buffer (BIOLINE, UK), 3 mM MgCl2, 0.04U BIOTAQ™ DNA polymerase (BIOLINE, UK), 0.2 mM dNTPs, and 0.8 µM of each primer.
In addition to template DNA, PCR reaction mixtures contained 1X PCR buffer (Bioline), 3 mM MgCl2, 0.04 U BIOTAQTM DNA polymerase (Bioline), 0.2 mM dNTPs, and 0.8 μM of each primer.
In addition to template switching, we found a high frequency of events in which one of the chromosomal loci became Ade+, reminiscent of earlier studies reporting "tripartite" recombination induced by a DSB on an ectopic template (Ray et al. 1989; Silberman and Kupiec 1994).
Similar(5)
In addition to template-based modeling, some researchers are now exploring a technique called "contact prediction".
For two NBS-LRR-encoding genes, At3g44400 and At4g33300, RACE was also performed using the total RNA extracted from flowers as templates in addition to the templates generated from leaf and young seedlings.
In addition to the template, support vector machines (SVMs) have also been incorporated to obtain geometrical parameters.
In contrast, preincubation of the substrate with purified Rad51 protein prior to its addition to the template promoted D-loop formation in a Rad51 concentration-dependent manner.
Another NGS technology, sequencing-by-synthesis (Illumina / Life Sciences), involves multiple rounds of reversible fluorescent dye-terminator addition to immobilized template by engineered polymerase, imaging, and dye and 3′ blocker removal [ 3].
Related(1)
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