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In addition, protocol timers cause numerous, unnecessary retransmissions.
Prompted by industry members, I have another grad student working to optimize tannin addition protocol for low tannin reds; 2009 was a perfect year for that project!
The method was also validated with a certified reference material (RTC-CRM818-050), wasch was quantified using a standard addition protocol.
To address the effect of mitochondrial isolation on mitochondrial H2O2 production under different energetic states, we again used a sequential substrate addition protocol.
To address the effect of experimental preparation on mitochondrial respiration, mitochondrial O2 consumption was measured during a sequential substrate addition protocol (schematically illustrated in Figure 3A) and normalized for a marker of mitochondrial content (complex IV activity).
The substrate addition protocol assessing O2 flux was added sequentially as follows, with each step interspersed with a period of stabilization between injections: 10 mM glutamate + 2 mM malate (GM), 2 mM adenosine di-phosphate (ADP), 10 µM succinate (SUCC), 10 µM cytochrome c, 10 µM antimycin A (AA), 5 mM ascorbate + 0.5 mM N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD).
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Two silica addition protocols were applied while maintaining the EISA synthesis route.
The optimized procedure was validated with powdered milk certified reference material, which was quantified using external calibration and standard addition protocols.
In addition, protocols can have other interesting properties, such as promoting collaboration between agents in a search for a better outcome.
In addition, latent addition protocols were utilised to assess the threshold increase at 0.2 ms, a marker of nodal persistent Na+ currents as SDTC measurements may be affected by other factors [24].
In addition, protocols have been proposed that allow for obtaining longer 'composite reads' from short read platforms [ 31].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com