Sentence examples for addition of substrate from inspiring English sources

Reactions were initiated by the addition of substrate and followed in 20 s intervals on a SpectraMax M5e (Molecular Devices) using 350 nm excitation and 460 nm emission wavelengths.

The plant population density at harvest and yield was improved by pesticide application and by addition of substrate in the seed row.

In the presence of saturating FAM, addition of substrate led to a slow, nearly stoichiometric reduction of C1 that was enzyme dependent, and independent of O−2 production.

Blanks of the samples were prepared by adding the TCA before the addition of substrate.

In these enzymatic assays, the reference cuvette contained all components except the substrate, and activity assay was initiated with the addition of substrate.

The solution mixture was maintained at 30 °C for 15 min using water bath with subsequent addition of substrate solution (5 μl).

The enzyme inhibitor solution was pre-incubated for 30 min at 37 °C before the addition of substrate to start the reaction.

The addition of substrate, OD measurement, and titer calculation were performed as described above.

After further washes liposomal binding to CD40 was assessed following the addition of substrate.

The addition of substrate, OD measurement, and titer determination were performed as described above.

Enzyme activity was initiated by addition of substrate in 100 µl assay buffer (final concentration 100 µM; see Table 2).