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where G a is the conductance at the ambient condition, G g is the conductance after the addition of solution, and ΔG represents the change of conductance after the addition of solution [21].
After the addition of solution #1, the mixture was kept stirring for 30 min, and then the white precipitate was collected by centrifugation, washed with DI water repeatedly until neutral pH, and dried at 60°C to approximately 70°C for a day.
Despite extensive efforts, we sometimes observed amplified products from negative control samples (PBS, distilled water, or no addition of solution).
Finally, the blot was developed by addition of solution containing 10 mM Tris (pH 7.4), 0.6 mg/ml di-amino benzidine and 100 µl hydrogen peroxide.
Approximately 2,000 cpm of the body-labeled wild-type C. botulinum intron and of the C.b.[Im1] variant purified by denaturing PAGE were annealed for 3 min at 50°C followed by 10 min at 32°C in 25 mM Na HEPES pH 7.5 [38], before addition of solution A or C (see Self-splicing assays section below) and further incubation for 5 min or 1.5 h at 32°C.
After addition of solution P2, gentle shaking and incubation for 5 min, P3 is added, the solutions are incubated at -20°C for 5 min, mixed again and after centrifugation the supernatant is pipetted to a new plate (A2).
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Sequentially, ZIF-67 rhombododecahedron nanoseeds were dispersed in methanol followed by the addition of solutions of Ni(NO3 2 and Co(NO3 2 in methanol.
By addition of solution-phase test ligands of pharmaceutical interest (which bind with different affinities to the target proteins), the target proteins, when bound to the test ligands, do not bind anymore or bind in a lower percentage to the beads, depending on their intrinsic test ligand affinities.
During the fixing and graded alcohol steps, the removal and addition of solutions was performed carefully and each added solution was allowed to equilibrate.
Thus, the addition of solutions of CMC and the uncharged hydroxypropyl methylcellulose (HPMC) to xanthan gum solutions was included to see if either hydrocolloid gave similar effects to saliva addition.
The bioactivity of the released drug was assessed by addition of solutions containing DEX released from GO/PPy films to primary astrocyte cultures and evaluation of the extent of interruption in cell proliferation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com