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A heuristic search was performed with the tree-bisection-reconnection (TBR) algorithm with random addition of sequences.
Heuristic searches for bootstrap analyses employed stepwise addition starting trees with simple addition of sequences and TBR branch-swapping.
Interestingly, the addition of sequences from −1065 to −1367 appears to be repressive to the transcriptional activity of the Nrf2 promoter (Figure 2B).
After manual addition of sequences obtained from other primates by PCR, all exon alignments were concatenated to produce the final alignment shown in Figure S1.
The MP reconstructions were conducted in PAUP* via heuristic searches with random addition of sequences (10000 replicates) and the tree-bisection-reconnection algorithm.
MP trees were obtained using the Close-Neighbour-Interchange algorithm [77] with search level 3 [76], in which initial trees were obtained by random addition of sequences (10 replicates).
Similar(10)
Moreover, the addition of sequence-structure permits the comparison of a much broader phylogenetic spectrum [68].
This inability to predict SoxS binding sites suggests that addition of sequence determinants is necessary for determining SoxS-mediated expression.
However, the addition of sequence data provides a means to create short-term results from inventories and at the same time generate data helpful to taxonomists.
For each library, addition of sequencing adaptors was performed in 700 μl PCR mixtures.
Moreover, the overall occurrence of SNPs increases with the addition of sequence information from new individuals or populations.
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